ABSTRACT
Background Certain metabolites are closely related to the occurrence and development of pulmonary fibrosis, and the related mechanism has not been fully elucidated. It is necessary to explore the trends of various metabolites and causes of pulmonary fibrosis. Objective To discuss the trends of publication and research hotspots of pulmonary fibrosis-related metabolites by bibliometrics. Methods With "pulmonary fibrosis" and "metabolites" in both Chinese and English as primary keywords, literature search was conducted through public online databases: PubMed, Web of Science, SinoMed, and CNKI. NoteExpress 3.0 and Excel 2019 were used to store and organize the collected literature. Analyses included publication year, number of papers, institution, country/region, and journal title. VOSviewer 1.6.10 was used for visual analysis. Keyword co-occurrence was analyzed by setting the minimum threshold for the occurrence of keywords to 5 times. Results The research on pulmonary fibrosis and associated metabolites in foreign language was earlier than that in Chinese language. Since the 1990s, the number of literature showed an increasing trend in both foreign and Chinese language literature. A total of 1 062 articles were published in foreign languages, of which 864 articles contained the authors’ address information. The authors in the United States published 340 articles, followed by China with 196 articles, and then Japan, Germany, and Italy. There were 728 relevant pieces of literature published in Chinese, 709 of which included the authors’ institution information and 350 institutions were involved. North China University of Science and Technology, Shanxi Medical University, Peking University, Zhengzhou University, China Medical University，and Soochow University were the top 6 by number of publication. A total of 255 Chinese journals published 728 Chinese articles, and among them 242 articles (33.24%) were published by 12 journals having published more than ten articles per journal. A total of 1062 articles were published in 609 foreign language journals, and among them 179 articles (16.85%) were published by 8 journals with more than 15 articles published by each journal. The results of keywords co-occurrence analysis suggested that pulmonary fibrosis in association with glucose metabolism, lipid metabolism, amino acid metabolism, nucleotide metabolism, and biological oxidation were the common themes studied at home and abroad. Conclusion The number of publications on pulmonary fibrosis and metabolites has been on the rise in recent years, and the research hotspots include glucose metabolism, lipid metabolism, amino acid metabolism, nucleotide metabolism, and biological oxidation.
ABSTRACT
Objective@#To establish and evaluate a microfluidic chip platform for the rapid diagnosis of post-neurosurgical bacterial infection. @*Methods@#The pathogens isolated from patients with post-neurosurgical bacterial infection in Beijing Tiantan Hospital Affiliated to Capital Medical University during 2007 and 2016 and the epidemiological data from China drug resistance monitoring network CHINET were analyzed retrospectively. Based on the retrospective data and the molecular epidemiological information of drug-resistant bacteria reported in the literature, target pathogens and drug resistance gene parameters were selected. The microbial identification parameters from 10 different bacteria, including Klebsiella pneumoniae, Acinetobacter baumannii, Staphylococcus epidermidis, Enterobacter cloacae, Staphylococcus aureus, Escherichia coli, Enterococcus faecium, Enterococcus faecalis, Stenotrophomonas maltophilia and Pseudomonas aeruginosa, and the parameters of 15 drug resistance genes, including mecA, vanA, vanB, aacC1, aadA1, bla CTX-M-1 , bla CTX-M-9 , bla GES-1 , bla OXA-23 , bla OXA-24 , bla OXA-58 , bla OXA-66 , bla KPC-2 , bla IMP-4 and bla VIM-2 , were selected for designing a microfluidic chip platform. Using MAIDI-TOF MS for bacterial identification, multiplex PCR for the detection of drug resistance genes, micro-broth dilution method for the detection of drug resistance phenotypes and ESBLs screening test as reference methods, 13 known bacteria were used to evaluate the preliminary performance of the established microfluidic chip platform, and 108 cerebrospinal fluid bacterial culture positive specimens were used to evaluate the clinical application value of the microfluidic chip platform. @*Results@#The identification rates of 13 known strains and the coincidence rate of drug resistance genes were 100%. The coincidence rate of identification results for 108 cerebrospinal fluid bacterial culture positive specimens between the microfluidic chip platform and the MALDI-TOF MS method was as high as 94.44%. The coincidence rates of drug resistance phenotype of carbapenems, oxacillin, vancomycin, ESBLs and genotype between the microfluidic chip platform and the micro-broth dilution method or ESBLs screening test were above 90%. @*Conclusion@#The established microfluidic chip platform is fast and accurate, and has application value in microbial identification and the prediction of drug resistance, which may be used as an important supplementary method in the diagnosis of post-neurosurgical bacterial infection.
ABSTRACT
Objective To discuss the diagnostic value of serum sialic acid for glioma.Methods A retrospective study was conducted.The levels of sialic acid in serum samples of 95 glioma patients, 175 patients with brain benign tumor and 400 normal persons from October 2014 to March 2015 were detected by automatic biochemistry analyzer using enzymic method.The SNK-q test and analysis of variance were used to compare the difference of the groups.By making receiver operating characteristic ( ROC) curve, the cut-off value, sensitivity, specificity, positive predictive value and negative predictive value were calculated to assess the diagnostic value of serum salivary acid.Then the cut-off value was validated by using the serums of 30 glioma patients and 30 normal persons who were out-patients and healthy controls.Results The levels of serum sialic acid in patients with gliomas, patients with brain benign tumor and healthy individuals were (0.66 ±0.14 ) g/L, (0.61 ±0.09 )g/L, (0.54 ±0.07 )g/L.The serum salivary acid of glioma patients were higher than brain benign tumor patients (q=6.74,P0.05) among the glioma patients of different grades (8 of gradeⅠ,32 of gradeⅡ,24 of grade Ⅲ,31 of grade Ⅳ).There was no significant difference between the low grade patients (gradeⅠandⅡ) and the high grade patients (gradeⅢandⅣ) (t=0.55, P>0.05), but the level of serum sialic acid of high grade group had an increasing trend than the low grade group.The area under the ROC curves was 0.79.The cut-off value of serum salivary acid for diagnosing glioma was 0.61 g/L.The sensitivity was 67.74%, and the specificity was 80.60%.The positive predictive value was 44.68%, and the negative predictive value was 90.76%.Then the cut-off value was used as a diagnostic criteria, and the detected results of 30 glioma patients and 30 normal persons showed that the sensitivity was 63.30% and the specificity was 83.30%.Conclusions The serum sialic acid has good specificity and negative predictive value for diagnosing glioma.It may be a valuable diagnostic marker.
ABSTRACT
Accurate knowledge,diagnosis and treatment of breast ductal carcinoma in situ(DCIS),are crucial in controlling the development of breast cancer.In the diagnosis phase,breast ultrasound is commonly used as a screening tool,and a clear diagnosis can be made by mammography.Meanwhile,serological tests contribute to the detection of DCIS in early stages.In the treatment,the optimal surgical operation method remains debatable.It is widely acknowledged that the radiotherapy of postoperative patients should become more individualized.In addition,corresponding endocrine therapy helps those ER positive patients to reduce the recurrence.In the development of DCIS to invasive cancer,there are changes in gene and protein expressions,which may be a potential direction for further research.